투고논문

Abstract Protein structure determination using NMR spectroscopy requires a suite of heteronuclear 3-D NMR experiments that can take a couple of weeks for completion. During the experiments, protein samples may suffer from slow degradation due to co-purifying proteases, which complicates and slows down the assignment procedure. Here we describe a practical protocol to avoid unwanted proteolysis during the experiment.

Keywords protease inhibitor, protein degradation, protein stability

* Address correspondence to: Jeong-Yong Suh, Department of Agricultural Biotechnology, Seoul National University, Seoul 08826, Republic of Korea, Tel: 82-2-880-4879; Fax: 82-2-877-4906; E-mail: jysuh@snu.ac.kr

* Erratum in J. Kor. Magn. Reson. Soc. Vol. 27, No. 2, 2023 (DOI: 10.6564/JKMRS.2023.27.2.016)